Method for determining cell membrane dielectric breakdown

Details Method for determining cell membrane dielectric breakdown Method for determining cell membrane dielectric breakdown

1982-09-28

1984-09-18

Turk, Arnold

Chemistry: analytical and immunological testing

Biological cellular material tested

204 1T, 324 65R, 436149, G01N 2704

Patent

active

044725060

ABSTRACT:
A method and apparatus which enables cell membrane dielectric breakdown to be determined. This determination is essential for the rapid inexpensive, reliable, in vitro quantification of lymphocyte tumor-cell cytotoxicity, lymphocyte membrane fluidity and the analysis of abnormal or tumor cells. The apparatus incorporates therein a cell dispenser used in conjunction with a DC electric field exposure tube. Electronic circuitry and its associated components process, acquire and store cell voltage signals as the cells are drawn through the electric field exposure tube. The procedure involves quantifying the breakdown voltage required to electrically collapse the cell membrane. The electrical collapse is termed a dielectric breakdown and is characterized by a reversible drop in the cell's resistivity.

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