Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Reexamination Certificate
2006-10-17
2006-10-17
Naff, David M. (Department: 1651)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
C424S400000, C435S004000, C435S041000, C435S243000, C435S257100, C435S946000
Reexamination Certificate
active
07122337
ABSTRACT:
A method of determining calciotropic activity of a preparation of algae of the genusPadina pavonica.The algae preparation may be in the form of a powder or an extract. The method consists of culturing human or animal osteoblasts in a culture medium containing calcium ions in a well plate. The preparation is added thereto and non-fixed calcium ions are eliminated in the culture medium. Further, the culture medium is acidified to destroy an extracellular matrix formed by the osteoblasts in the culture medium. Thereafter, filtering of the culture medium is carried out and then a concentration of fixed calcium ions in the osteoblasts is determined and compared with a calibration scale of a known activator of the fixation of calcium or in the presence of an inhibitor of the fixation of calcium or both.
REFERENCES:
patent: 2001-139419 (2001-05-01), None
Muserlian Charles A.
Naff David M.
Patrinove SC
Texinfine SA
Ware Deborah K.
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