Method for determining biophysical properties

Details Method for determining biophysical properties Method for determining biophysical properties

2005-06-13

2009-10-27

Whisenant, Ethan (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C536S023100, C536S024300

Reexamination Certificate

active

07608397

ABSTRACT:
The invention is a method for detecting a change in the conformational or energetic state of a molecular species, comprising the steps of: (i) immobilizing the molecular species, under conditions suitable for the reaction to occur; (ii) contacting at least part of the molecular species with a localized electromagnetic field; and (iii) detecting a change in dielectric constant during or after the reaction, to thereby detect a change in the conformational or energetic state of the molecular species.

REFERENCES:
patent: WO 1999/05315 (1999-02-01), None
patent: WO 2005/007887 (2005-01-01), None
Russo et al., Atomic force sensing of light-induced protein dynamics with microsecond time resolution in bacteriorhodpsin and photosynthetic reaction centers. Journal of Structural Biology 119 : 158-164 (1997).
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Hansen, Karolyn M. et al. “Cantilever-Based Optical Deflection Assay for Discrimination of DNA Single-Nucleotide Mismatches”, Apr. 1, 2001,Anal. Chem.,vol. 73, No. 7, pp. 1567-1571.
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Ros, Robert et al. “Single molecule force spectroscopy on ligand-DNA complexes: from molecular binding mechanisms to biosensor applications”, Aug. 26, 2004,Journal of Biotechnology,vol. 112, No. 1-2, pp. 5-12.
Yi, Mingqiang et al. “Theoretical and experimental study towards a nanogap dielectric biosensor”, Jan. 15, 2006,Biosensors&Bioelectronics, Elsevier Science Publishers, Barking, GB, vol. 20, No. 7, pp. 1320-1326.

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