Method for determining bacterial endotoxin and kit therefor

Details Method for determining bacterial endotoxin and kit therefor Method for determining bacterial endotoxin and kit therefor

1982-11-15

1985-01-22

Shapiro, Lionel M.

Chemistry: analytical and immunological testing

For preexisting immune complex or auto-immune disease

435 4, 435810, 435 23, 435 24, 436175, G01N 3316, C12Q 100, C12Q 138, C12Q 136

Patent

active

044952945

ABSTRACT:
Bacterial endotoxin in an assay sample is determined by an amoebocyte lysate component of horseshoe crab in the presence or absence of a substrate for endotoxin determination by using an assay sample in the liquid state obtained by treating said assay sample with an endotoxin-free acid having a pKa.sup.25.degree. C. value of not more than 3 at a pH of not more than 3; and a kit therefor, a receptacle containing (a) an endotoxin-free acid having a pKa.sup.25.degree. C. value of not more than 3 e.g. perchloric acid, trichloroacetic acid, nitric acid, for treating the assay sample and a receptacle containing (b) an amoebocyte lysate component or horseshoe crab. The acid treatment of the assay sample results in improved accuracy, reliability and reproducibility of the bacterial endotoxin determination.

REFERENCES:
patent: 4038147 (1977-07-01), Reno
patent: 4067776 (1978-01-01), Khan
patent: 4188264 (1980-02-01), Iwanaga et al.
patent: 4370413 (1983-01-01), Neeman et al.
Reinhold, R. B. et al., Proc. Soc. Exp. Biol. and Med. vol. 137; 334-340, 1971.
Tamura, H. et al., "Perchloric Acid Treatment of Human Blood for Quantatative Endotoxin Assay Using Synthetic Chromogenic Substrate for Horseshoe Crab Clotting Enzyme" Chem. Abst. 97, 157505u, 1982.

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