Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1999-02-23
2000-10-31
Leary, Louise N.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 4, 4352831, 435 29, 435 30, C12Q 118, C12Q 100, C12Q 102, C12Q 124
Patent
active
061400696
ABSTRACT:
A method and apparatus for determining the minimum inhibitory concentration of an antibiotic for a target microorganism is provided. The method includes the steps of: (a) providing a microorganism growth medium; (b) providing a sensible reagent, which includes an antibiotic mixed with a marker, the marker having a signal with a magnitude proportional to the marker's concentration; (c) incorporating the reagent into the growth medium, in a manner that creates a gradient of concentrations of the antibiotic and marker within the growth medium; (c) inoculating the growth medium with the target microorganism; (d) incubating the inoculated growth medium for a period of time sufficient for the target microorganism to grow a detectable amount on a first section of growth medium; (e) determining a growth boundary between the first section of growth medium having detectable target microorganism growth and a second section having substantially no detectable target microorganism growth; (f) measuring the signal magnitude at the growth boundary; and (g) determining a minimum inhibitory concentration of the antibiotic using the measured signal magnitude.
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Leary Louise N.
Levine Robert A.
Wardlaw Partners, LL.P.
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