Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1991-04-02
1994-08-16
Kepplinger, Esther L.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 792, 435 793, 436501, 436512, 436815, 436816, 436817, 436901, C12Q 100, G01N 3353, G01N 33566, G01N 33563
Patent
active
053386590
ABSTRACT:
The precision of identification of analyte composition in a sample, where the possible analytes cross-react with specific binding reagents, is enhanced by applying pattern recognition techniques. Samples to be tested are reacted with a panel of specific binding reagents reactive with the set of analytes to be tested to obtain a pattern of reactivity with respect to each analyte at a given concentration. This results in a databank of "CRIM profiles" for known concentrations of each analyte. This databank is stored in a computationally accessible form, which then can be matched against CRIM patterns obtained by testing unknown samples. In one embodiment, each CRIM pattern obtained is plotted as a single point in n-dimensional space, wherein n represents the number of specific binding reagents in the panel. Because a different CRIM profile as a function of concentration is obtained with each analyte, a pattern of points in n-dimensional space can be constructed for various concentrations of each individual member. Mathematical techniques can be used to project the pattern from n-dimensional space to reduce dimensionality. The location of a point yielded by a sample of unknown concentration can then be matched against those for predetermined known compositions to determine best estimate.
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Ambler Stuart M.
Kauvar Lawrence M.
Kepplinger Esther L.
Preston David R.
Terrapin Technologies, Inc.
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