Method for determining an index of binding protein content of bl

Details Method for determining an index of binding protein content of bl Method for determining an index of binding protein content of bl

1974-05-31

1976-06-01

Serwin, R. E.

Chemistry: physical processes

Physical processes

Crystallization

232303, 252408, 424 1, G01N 3316

Patent

active

039604926

ABSTRACT:
A method for determining an index of binding proteins in a serum sample comprising the steps of: 1. Forming a mixture of a) the serum sample, having ligand binding sites, b) a quantity (usually a trace amount) of radioactive ligand and c) an amount of non-radioactive ligand such that the total amount of ligand (radioactive and non-radioactive) is in excess of the ligand binding sites available in the serum sample; 2. Contacting the mixture with a quantity of a binder having a preferential affinity for the ligand; and 3. Thereafter determining the distribution of the radioactive ligand such as by measuring the radioactivity of the binder.
The method is particularly applicable for determining thyroxine binding protein content in serum.
The method is also applicable to determine an index of serum free thyroxine as determined by the ratio of the concentration of thyroxine to the concentration of thyroxine binding proteins.

REFERENCES:
patent: 3376114 (1968-04-01), Eberle
patent: 3451777 (1969-06-01), DiGiulio
patent: 3519390 (1970-07-01), Dickey et al.
patent: 3646346 (1972-02-01), Catt
patent: 3666854 (1972-05-01), Eisentraut
patent: 3716632 (1973-02-01), Fader et al.
patent: 3730684 (1973-05-01), Demetriou
patent: 3743482 (1973-07-01), Eisentraut
patent: 3745211 (1973-07-01), Brown et al.
patent: 3776698 (1973-12-01), Eisentraut
Clark, et al., J. Clin. Endocr. vol. 25, 1965, pp. 39-45.

Affiliated with

Also associated with

Rating

Method for determining an index of binding protein content of bl does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for determining an index of binding protein content of bl, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for determining an index of binding protein content of bl will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2400939