Method for determining allergic sensitivity

Chemistry: analytical and immunological testing – Involving iga – igd – ige – or igm

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436 34, 436 50, 436 63, 436 69, 436 70, 436517, 73 641, 422 73, 424 91, 435 13, G01N 3349

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active

046297065

ABSTRACT:
A method for determining allergenic sensitivity of a mammal is disclosed. The method involves measuring the time required for hemostasis of a blood sample with an allergen in the presence of a blood clotting inhibitor and comparing the time required for hemostasis in the presence of a blood clotting inhibitor and the absence of the allergen. The sensitivity to the allergen is determined by determining the difference in time required for hemostasis.

REFERENCES:
patent: 3720760 (1973-03-01), Bennich et al.
patent: 4581920 (1986-04-01), Volkl
J. Immunol., 115:525-532 (1975), R. N. Pinckard, et al., "IgE-Induced Blood Coagulation Alterations in the Rabbit: Consumption of Coagulation Factors XII, XI & IX In Vivo".
Clinical Research, 25:361A (1977), A. P. Kaplan, et al., "Human Anaphylaxis: A Study of Mediator Systems".
J. Immunol., 123:2835-2841 (1979), L. M. McManus, et al., "Platelet Activating Factor (PAF) Induced Release of Platelet Factor 4 (PF4) In Vitro & During IgE Anaphylaxis in the Rabbit".
J. Lab. Clin. Med., 89(6):1306-1313 (1977), J. Blakely, et al. "An In Vitro Measurement of Bleeding Time".
J. Lab. Clin. Med., 81:291 (1973), D. A. Levy, et al., "A Microassay for Studying Allergic Histamine Release from Human Leukocytes Using an Enzymic-Isotopic Assay for Histamine".

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