Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase
Reexamination Certificate
2002-02-14
2008-03-04
Foley, Shanon (Department: 1642)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving transferase
C435S004000, C435S007100
Reexamination Certificate
active
07338774
ABSTRACT:
A method for determining the activity of a cell cycle regulatory factor comprising the steps of:preparing a sample for measuring a cyclin-dependent kinase/cyclin complex from living cells;reacting adenosine 5′-O-(3-thiotriphosphate) (ATP-γS) with a substrate for the cyclin-dependent kinase in presence of the sample in order to introduce a monothiophosphate group into a serine or threonine residue of the substrate;labeling the substrate by coupling a labeling fluorophore or a labeling enzyme with a sulfur atom of the introduced monothiophosphate group;measuring the amount of fluorescence from the labeling fluorophore labeling the substrate, or reacting the labeling enzyme labeling the substrate with a substance which generates an optically detectable product by reaction with the labeling enzyme and optically measuring the amount of the generated product; andcalculating the activity of the cyclin-dependent kinase from the measured amount of fluorescence or the measured amount of the generated product with reference to a pre-produced reference curve.
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Ishihara Hideki
Tada Sachiyo
Yamasaki Masatoshi
Yoshida Tomokazu
Foley Shanon
Goddard Laura B
Sysmex Corporation
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