Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase
Patent
1978-08-14
1980-12-23
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving transferase
435 26, 435810, C12Q 152, C12Q 132
Patent
active
042411793
ABSTRACT:
An improvement in a method for determining glutamate oxalacetate transaminase or glutamate pyruvate transaminase present in a biological fluid, in which method L-glutamate is produced from alpha-ketoglutarate by transamination in the presence of the transaminase in the fluid, and the L-glutamate is oxidatively deaminated in the presence of glutamate dehydrogenase with the simultaneous production of reduced beta-nicotinamide adenine dinucleotide in an amount proportional to the content of the transaminase in the fluid, such fluid also containing an endogenous substance which is oxidized in the presence of glutamate dehydrogenase with the simultaneous production of reduced beta-nicotinamide adenine dinucleotide, thereby interfering with the determination. The improvement for obviating the interference caused by the endogenous substance includes oxidizing the endogenous substance in the presence of glutamate dehydrogenase with the simultaneous reduction of beta-nicotinamide adenine dinucleotide, oxidizing the reduced beta-nicotinamide adenine dinucleotide in the presence of lactate dehydrogenase with the simultaneous reduction of pyruvate to lactate, thereafter inhibiting the lactate dehydrogenase activity, and thereafter producing a quantity of L-glutamate from alpha-ketoglutarate by the aforesaid transamination for oxidative deamination thereof and simultaneous production of reduced beta-nicotinamide adenine dinucleotide in an amount proportional to the content of the transaminase in the fluid. A reagent combination is employed for the determination.
REFERENCES:
patent: 3956069 (1976-05-01), Allain et al.
patent: 3985621 (1976-10-01), Maruyama et al.
patent: 4019961 (1977-04-01), Klose et al.
patent: 4024021 (1977-05-01), Strauropoulos et al.
patent: 4086142 (1978-04-01), Huang et al.
Bucolo Giovanni
Madappally Mathew M.
Coulter Electronics Inc.
Wiseman Thomas G.
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