Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase
Patent
1978-07-26
1980-12-30
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving transferase
435 16, 435 18, 435 22, 435 25, 435 26, 435184, 435810, C12Q 152, C12Q 134, C12Q 140, C12Q 132
Patent
active
042424461
ABSTRACT:
An improvement in a method for determining a substance present in a biological fluid by oxidizing a compound produced in the course of the determination in the presence of a dehydrogenase enzyme with the simultaneous production of reduced beta-nicotinamide adenine dinucleotide in an amount proportional to the content of the substance in the fluid, which fluid also contains an endogenous material which or a derivative of which produced during the determination likewise is oxidized in the presence of the enzyme with the simultaneous production of reduced beta-nicotinamide adenine dinucleotide, thereby interfering with the determination, which improvement involves first carrying out the oxidation-reduction reaction of the endogenous material or its derivative, then oxidizing the resulting reduced beta-nicotinamide adenine dinucleotide in the presence of lactate dehydrogenase with the simultaneous reduction of pyruvate to lactate, thereafter inhibiting the lactate dehydrogenase enzymatic activity, and thereafter producing a quantity of the aforesaid compound produced in the course of the determination for conducting the oxidation-reduction reaction therewith without interference caused by the endogenous material or the lactate dehydrogenase. Substances which may be determined include alpha-amylase, transaminases, and triglycerides. A two-reagent combination is employed for the determination.
REFERENCES:
patent: 3838011 (1974-09-01), Hngen et al.
patent: 3925018 (1975-12-01), Saunders
patent: 3956069 (1976-05-01), Allain et al.
patent: 4000042 (1976-12-01), Adams
patent: 4086142 (1978-04-01), Huang et al.
Madappally et al., "Enzymatic Determination of Serum Amylase", Clin. Chem., vol. 22, No. 7, (1976) p. 1164.
Proelss et al., "New, Simple Maltogenic Assay for Mechanized Determination of Alpha-Amylase Activity in Serum and Urine", Clin. Chem., vol.21, No. 6 (1975) pp. 694-702.
Bucolo Giovanni
Madappally Mathew M.
Coulter Electronics Inc.
Wiseman Thomas G.
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