Method for determining a presence or absence of a nonparaffinoph

Details Method for determining a presence or absence of a nonparaffinoph Method for determining a presence or absence of a nonparaffinoph

1998-07-24

1999-11-09

Leary, Louise N.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 29, 435 4, 435 23, 435183, 435863, 435872, 435874, 435875, 435876, 435877, 435963, 435970, 536 231, C12Q 104, C12Q 102, C12Q 100

Patent

active

059812109

ABSTRACT:
The method of the invention involves providing a first receptacle and a second receptacle. The first receptacle contains a sterile aqueous broth and the second receptacle contains an aqueous broth including a carbon source. The method then includes placing into the first receptacle a first support surface having a paraffin wax coating thereon and placing into the second receptacle a second support surface having a hydrophobic material coating thereon. A body specimen, such as sputum, is then introduced into each of the first and second receptacles. The presence of a nonparaffinophilic hydrophobic microorganism in the body specimen is determined by observing (i) a lack of microorganism growth on the paraffin coated material of the first support surface and (ii) a presence of microorganism growth on the hydrophobic material coating of the second support surface. The presence of the nonparaffinophilic hydrophobic microorganism can be further confirmed by performing a DNA extraction. An associated DNA extraction procedure is also provided.

REFERENCES:
patent: 5153119 (1992-10-01), Ollar
patent: 5472877 (1995-12-01), Ollar
patent: 5569592 (1996-10-01), Ollar
patent: 5639675 (1997-06-01), Felder et al.
patent: 5641645 (1997-06-01), Felder et al.
patent: 5654194 (1997-08-01), Felder et al.
patent: 5663056 (1997-09-01), Ollar et al.
patent: 5668010 (1997-09-01), Felder et al.
patent: 5677169 (1997-10-01), Ollar et al.
patent: 5846760 (1998-12-01), Ollar

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