Method for determination of triglycerides and glycerol

Details Method for determination of triglycerides and glycerol Method for determination of triglycerides and glycerol

1974-03-20

1977-01-04

Monacell, A. Louis

Textiles: fiber preparation

23230B, C12K 104, G01N 3100

Patent

active

040010899

ABSTRACT:
A novel reagent and method for colorimetrically determining the concentration of triglycerides in biological fluids, and for rapid determination of glycerol are described. Triglycerides are hydrolyzed in methanolic base to glycerol, and glycerol content is determined colorimetrically.

REFERENCES:
patent: 3703591 (1972-11-01), Bucolo et al.
patent: 3791931 (1974-12-01), Thum et al.
Otto Wieland, Methods of Enzymatic Analysis, pp. 211-214, Academic Press, New York and London, 1965.
T. P. Hilditch et al., The Chemical Constitution of Natural Fats, John Wiley & Sons Inc., N.Y., 1964.
S. P. Colowick et al., "Methods in Enzymology," XIV, pp. 627-630, Academic Press, N.Y. and London, 1969.
S. Klotzsch et al., "An Automated Colorimetric Method for the Specific Determination of Serum Triglycerides," Abstract CC31, Technicon International Congress, Advances in Automated Analysis, Technicon Instruments Corporation, Tarrytown, N.Y., (1972).

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