Method for determination of the presence of antibiotics

Details Method for determination of the presence of antibiotics Method for determination of the presence of antibiotics

1974-05-22

1976-03-02

Monacell, A. Louis

Textiles: fiber preparation

C12K 104

Patent

active

039416585

ABSTRACT:
A method for the rapid determination of the presence or absence of residues of antibiotic such as penicillin, in liquids, for example milk, and meat comprising introducing spores of a microorganism which possesses a high sensitivity for the antibiotic to be determined into an agar medium so that the spores are prevented from starting to germ due to lack of nutrients, but stay alive, and being sufficiently heavily inoculated that after addition of nutrients and incubation at or near optimal temperature in a short time sufficient growth has proceeded so that growth is either observable visually or its indicated by an indicator added to the agar medium, the said spore culture being allowed to solidify in upright test vessels having a cross-sectional dimension sufficiently small to require as little of the agar medium as possible but sufficiently large to enable a correct determination of inhibition of growth of the microorganism to be made, and having a height so that the vessel can contain a sufficient amount of medium and a sufficient amount of sample of liquid to be tested, placing on the agar surface nutrients required for growth, followed, if desired, after a pre-incubation period, by addition of a predetermined amount of sample liquid to be tested to the test vessel, and incubating the contents of the test vessel at or near optimal temperature for a predetermined time so that the extent of growth or inhibition of growth of the microorganism read in a vertical sense, indicates absence or presence of the antibiotic.

REFERENCES:
patent: 2967132 (1961-01-01), Sacks
patent: 3063916 (1962-11-01), Kosikowski
patent: 3126325 (1964-03-01), Poole
H. Mol, "A Rapid and Sensitive Test for the Detection of Penicillin in Milk, Using Test Plates Which Can Be Kept in Stock Ready for Use," Neth. Milk Dairy J. 23 (1969) pp. 153-163.

Affiliated with

Also associated with

Rating

Method for determination of the presence of antibiotics does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Method for determination of the presence of antibiotics, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for determination of the presence of antibiotics will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1276650