Method for determination of the amount of either phosphotyrosine

Details Method for determination of the amount of either phosphotyrosine Method for determination of the amount of either phosphotyrosine

1995-06-06

1997-11-11

Alexander, Lyle A.

Chemistry: analytical and immunological testing

Peptide, protein or amino acid

436172, 436103, C07K 113

Patent

active

056863101

ABSTRACT:
Traditional methods for measuring the phosphorylation state of cellular proteins have relied on .sup.32 P-orthophosphate labeling of cells in culture. The problems associated with radioactive isotopes have therefor prohibited phosphorylation studies on whole animals and humans. The present invention provides a unique technique that allows quantitative attachment of probes (e.g. fluorophors or biotin) to phospho-proteins and peptides by activating phospho-amino acids. the phosphorylation state of cellular proteins derived from human tissues can now be measured. This method can be used for early detection of cancer and diabetes and also for the identification of pharmaceutical agents.

REFERENCES:
patent: 5185243 (1993-02-01), Ullman et al.
patent: 5284558 (1994-02-01), Linhardt et al.
BIOSIS 95:166168, Fadden et al. (abstract only), 1995.
BIOSIS 92:68405, Byford (abstract only), 1992.
BIOSIS 88:393314, Odani et al. (abstract only), 1988.

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