Method for determination of percentage T cell content of lymphoc

Image analysis – Histogram processing – For setting a threshold

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364416, 377 10, G06K 900

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045625930

ABSTRACT:
A method for determining the percentage T-cell content of lymphocyte, which comprises scanning a test specimen with a pickup device, discriminating a lymphocyte from other cells contained in said specimen from the output level and regional information of the image signals transmitted from said pickup device, searching the data marginal to those of the cell discriminated as lymphocyte, to detect pixels corresponding to the image signal output level different from that corresponding to the lymphocytes or background, and discriminating a T-cell from other lymphocytes from the number of detected pixels.

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Lehninger, Biochemistry, the Molecular Basis of Cell Structure and Function, second edition, Chapter 35, pp. 1004-1006.

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