Method for determination of hydrogen sulfide or sulfide ion...

Chemistry: analytical and immunological testing – Sulfur containing – Organic or sulfhydryl containing

Reexamination Certificate

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C436S119000, C436S164000, C435S004000, C435S232000, C435S130000

Reexamination Certificate

active

06969613

ABSTRACT:
The present invention provides a method for quantitatively determining hydrogen sulfide or sulfide ions conveniently with high sensitivity, which comprises adding to a sample containing hydrogen sulfide or sulfide ions, metal ions or a compound which liberates said metal ions and a metal indicator which reacts with the metal ions and resultingly undergoes color development, wherein the color development is accelerated or inhibited by the hydrogen sulfide or sulfide ions; and measuring the degree of color development of the metal indicator. The present invention further provides a method for quantitatively determining a specific substance, which comprises adding to a sample containing a specific substance, a component which acts on the specific substance so that the specific substance forms hydrogen sulfide or sulfide ions, metal ions or a compound which liberates said metal ions, and a metal indicator which reacts with the metal ions and resultingly undergoes color development, wherein the color development is accelerated or inhibited by the hydrogen sulfide or sulfide ions; and measuring the degree of color development of the metal indicator.

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P. Sharma et al, “Studies on Metal-Metal Sulphide Electrodes: Part I—Application of Arsenic-Arsenic Sulphide, Antimony-Antimony Sulphide & Molybdenum-Molybdenum Sulphide Electrodes in the Quantitative Determination of Sulphide Ion”,Indian Journal of Chemistry, vol. 21A, pp. 259-263 (Mar. 1982).
A. Svenson, “Rapid and Sensitive Spectrophotometric Method for Determination of Hydrogen Sulfide with 2, 2′-Dipyridyl Disulfide”,Anal. Biochem., vol. 107, pp. 51-55 (1980).
M.F. Mousavi et al, “Spectrophotometric Determination of Trace Amounts of Sulfide Ion Based on Its Catalytic Reduction of Toluidine Blue”,The Chem. Soc. of Japan, vol. 65, No. 10, pp. 2770-2772 (1992).
I.G. Gökman et al, “Determination of Selenium in Biological Matrices Using a Kinetic Catalytic Method”,Analyst, vol. 119, pp. 703-708 (1994).

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