Method for determination of HIV specific antibodies

Details Method for determination of HIV specific antibodies Method for determination of HIV specific antibodies

1990-01-19

1991-03-19

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

530317, 530329, 435 75, 435974, C12Q 170, A61K 3702, C07K 700

Patent

active

050010492

ABSTRACT:
The invention relates to a method for determining HIV (human immunodeficiency virus) specific antibodies. A sample is incubated with a solid phase, to which streptavidin is bound in a way to make 0.1-2.5 .mu.g streptavidin per ml of reaction volume available for binding, a biotinylated polypeptide, and a labelled receptor which binds to HIV specific antibodies. The biotinylated polypeptide contains at least one of the amino acid sequences ##STR1## and is from 7 to 50 amino acids long. Following complex formation onto the solid phase, solid and liquid phase are separated, and label is measured in one of these.

REFERENCES:
patent: 4746604 (1988-05-01), Mowshowitz
Gnann, J. W. Science, 237: 1346-1349.

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