Method for determination of glucose concentration in whole blood

Details Method for determination of glucose concentration in whole blood Method for determination of glucose concentration in whole blood

1992-01-16

1993-12-21

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving glucose or galactose

435 25, 435 29, 436 63, 436 95, C12Q 126, C12Q 102, G01N 3348, G01N 3300

Patent

active

052720608

ABSTRACT:
There is provided a method for the determination of a glucose concentration in a whole blood utilizing a biosensor. A correction of the measured glucose concentration for dilution error introduced by the solid component of blood cells is calculated based on the change in glucose concentration measured before and after significant glucose has diffused from blood cells into the buffer used to dilute the sample. Thus, the need to centrifuge blood samples to obtain a cell-free serum sample for glucose determination is eliminated.

REFERENCES:
patent: 4547280 (1985-10-01), Karasawa et al.
patent: 5168046 (1992-12-01), Hamamoto et al.
Abstract of Japanese Patent Document 59-168371; Yumiko Abe et al; Sep. 22, 1984.
Extended Abstracts/Electrochemical Society, vol. 87, No. 2, 1987, p. 2276; Genshaw et al. "Whole Blood Glucose Enqyme Electrode".
Clin. Chem. vol. 25, No. 4, 1979, pp. 531-534; Meites et al.; "Preservation, Distribution, and Assay of Glucose in Blood, With Special Reference to the Newborn".
Biological Abstracts, vol. 82, No. 10, 1986, p. 605, T. Kanluan et al.; "Comparison of Plasma and Whole Blood Glucose Determination".

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