Method for determination of enzyme activity

Details Method for determination of enzyme activity Method for determination of enzyme activity

1986-06-17

1988-09-20

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving blood clotting factor

435 19, 435 23, 435184, C12Q 156, C12Q 144, C12Q 138, C12N 999

Patent

active

047725532

ABSTRACT:
This invention relates to a method for determining an enzyme-, an enzyme inhibitor-, an enzyme activator-, or zymogen-activity, which comprises allowing the enzyme to react with a substrate of the formula ##STR1## (wherein R.sub.1 represents an amino acid of an oligopeptide combined in the form of ester through the C-terminal carboxyl group of said amino acid or oligopeptide and R.sub.2 represents a hydrogen or bromine atom) to hydrolyze the substrate, then allowing the hydrolysis product of the formula ##STR2## to react with a FR-ITR salt [Fast Red-ITR salt (N,N'-diethyl-4-methoxymetanilamide diazonium salt)] to form a pigment, and determining the pigment.

REFERENCES:
patent: 3862011 (1975-01-01), Smith
patent: 4257940 (1981-03-01), Fujii et al.
patent: 4308201 (1981-12-01), Fujii
patent: 4308202 (1981-12-01), Fijii et al.
Sanders et al., Clin. Chim. Acta, 89: 421-427, "Interference with the Kinetic Determination of Acid Phosphatase", (1978).
Thielemann, Chemical Abstracts, 81:72336j, p. 627 (1974).
Holzmann et al, J. Immunol. Meth. 60:359-367, Jun. 10, 1983.

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