Chemistry: analytical and immunological testing – Nitrogen containing
Patent
1988-11-03
1990-04-17
Richman, Barry S.
Chemistry: analytical and immunological testing
Nitrogen containing
436109, 436161, 436164, 436177, G01N 3300
Patent
active
049180240
ABSTRACT:
The present invention is a method for determination of dicyanodiamide in a plant matrix, i.e. in a plant or a part of a plant. It involves a series of steps including extracting any dicyanodiamide from the plant matrix as well as purifying the dicyanodiamide obtained of interfering substances. Next, the dicyanodiamide obtained is reacted with 3-hydroxy-2-butanone in a non-aqueous, alcoholic hydrochloric acid medium to form 2-guanidino-4,5-dimethyloxazole, and then any 2-guanidino-4,5-dimthyloxazole formed is isolated using liquid-liquid extraction. Finally, any isolated 2-guanidino-4,5-dimethyloxazole is purified by reverse phase high pressure liquid chromatography (HPLC) and the amount of 2-guanidino-4,5-dimethyloxazole is determined spectrophotometrically at a wavelength of 260 nm.
REFERENCES:
patent: 4692415 (1987-09-01), Rust
Buyske et al., "Spectrophotometric Determination of Cyanamide", Analyt. Chem., 32, 1798-1800, 1960.
Nieman et al., "Reaction Rate Method for Determining Trace Concentrations of Cyanamide", Analyt. Chem., 48, 899-902, 1976.
Glynn Kenneth P.
Richman Barry S.
Vassilatos Thalia P.
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