Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase
Patent
1991-07-09
1994-04-12
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving transferase
435 25, 435 26, 435 23, 435 16, 435 14, 435 4, C12Q 148, C12Q 126, C12Q 137, C12Q 152
Patent
active
053025130
ABSTRACT:
The present invention relates to a method for quantitative determination of NAD(P)H derived from a specific component, which comprises converting NAD(P)H present in a sample or formed from components other than the objective component by reactions into NAD(P) by the action of glutathione of oxidation type and glutathione reductase; decomposing the remaining glutathione of oxidation type by the action of .gamma.-glutamyl transpeptidase in the presence or in the absence of a mercapto compound; forming NAD(P)H from the component to be determined in the sample utilizing an NAD(P)H-forming reaction system; and quantitatively determining NAD(P)H.
According to the method of the present invention, amylase activity, etc. in vital components containing maltose and glucose can be determined accurately.
REFERENCES:
patent: 3627645 (1969-07-01), Grassetti
Zubay, "Biochemistry"; pp. 878-882, 1983.
Hagen et al., Can J. Biochem. and Physiology, vol. 40, pp. 1129-1139 (1962).
Miike Akira
Tatano Toshio
Kyowa Medex Co., Ltd.
Leary Louise N.
Wityshyn Michael G.
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