Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase
Patent
1994-05-25
1997-03-04
Kight, John
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving hydrolase
435 14, 435 15, 435 18, 435 26, 435 4, 435832, 436 74, 436 63, C12Q 140, C12Q 154, C12N 100, G01N 3320
Patent
active
056078382
ABSTRACT:
A method for determining .alpha.-amylase activity which involves bringing a sample into contact with an .alpha.-glucosidase in the presence of an .alpha.-amylase substrate and optically determining a liberated label, the substrate being a maltooligosaccharide composed of at least 3 glucose units, whose reducing terminal glucose is bonded to an optically measurable label at the 1-position by .alpha.-glucoside linkage or .beta.-glucoside linkage, and whose non-reducing terminal glucose is modified by a substituent other than glucose, and the .alpha.-glucosidase being substantially capable of acting on glucose to which the label is bonded at the 1-position by .alpha.-glucoside linkage and on all maltooligosaccharides having 2 to 7 glucose units; and a reagent for determining .alpha.-amylase activity comprising the .alpha.-glucosidase and said .alpha.-amylase substrate. The present invention permits, in the determination of .alpha.-amylase activity, efficient auxiliary action of adjuvant enzyme, a determination with good sensitivity, and little reagent blank reaction.
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Emi Shigenori
Hattori Shizuo
Sogabe Yukihiro
Yamamoto Yoshihiro
Kight John
Leary Louise N.
Toyo Boseki Kabushiki Kaisha
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