Method for determination of acid phosphatase activity using 4-ac

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving hydrolase

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435 19, 558198, C12Q 144

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052272915

ABSTRACT:
Phosphoric acid derivatives represented by formula (I): ##STR1## wherein X is a halogen and R is --(CH.sub.2).sub.n CH.sub.3 (n=0 to 3), or salts thereof are stable to non-enzymatic hydrolysis and are capable of specifically reacting with acid phosphatase. Therefore, the activity of acid phosphatase in the sample can be determined extremely accurately by reacting said compound with a sample containing acid phosphatase and quantitatively determining the reaction product by colorimetry.

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