Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-03-10
1996-05-14
Zitomer, Stephanie W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
935 77, 935 78, 435 911, C12Q 168, C12P 1934
Patent
active
055166415
ABSTRACT:
A kit is disclosed for a method for detecting the presence of a target polynucleotide sequence. The kit comprises a first polynucleotide sequence and a second polynucleotide sequence complementary to non-contiguous portions of a target polynucleotide sequence, which first and second sequences are covalently attached when they are hybridized to the target sequence. The presence of the covalently attached first and second sequences is related to the presence of the target polynucleotide sequence. The invention may be applied to target polynucleotide sequences in DNA or RNA. Specific target polynucleotide sequences of interest will frequently be characteristic of particular microorganisms, viruses, viroids, or genetic characteristics, including genetic abnormalities.
REFERENCES:
patent: 4423153 (1983-12-01), Ranney et al.
patent: 4480040 (1984-10-01), Owens et al.
patent: 4486539 (1984-12-01), Ranki et al.
patent: 4490472 (1984-12-01), Gottlieb
patent: 4563419 (1986-01-01), Ranki et al.
patent: 4599303 (1986-07-01), Yabusaki et al.
patent: 4647529 (1987-03-01), Rodland et al.
patent: 4675283 (1987-06-01), Roninson
patent: 4677054 (1987-06-01), White et al.
patent: 4683194 (1987-07-01), Saiki et al.
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4716106 (1987-12-01), Chiswell
patent: 4725536 (1988-02-01), Fritsch et al.
patent: 4731325 (1988-03-01), Palva et al.
patent: 4775619 (1988-10-01), Urdea
patent: 4851331 (1989-07-01), Vary et al.
patent: 4868104 (1989-09-01), Kurn et al.
patent: 4883750 (1989-11-01), Whiteley et al.
patent: 4994368 (1991-02-01), Goodman et al.
Gardner, Biotechniques, vol 1: pp. 38-41, (1983) "Non-radioactive DNA Labeling: Detection of Specific DNA and RNA Sequences on Nitrocellulose and in situ Hybridizations".
Langer, et al., Proc. Natl. Acad. Science USA, vol. 78:11, pp. 6633-6637, (Nov. 1981) "Enzymatic synthesis of biotin-labeled polynucleotides: Novel nucleic acid affinity probes".
Brigati, et al., Virology, vol. 126: pp. 32-50, (1983) "Detection of Viral Genomes in Cultured Cells and Paraffin-Embedded Tissue Sections Using Biotin-Labeled Hybridization Probes".
Lewin, Science, vol. 221, p. 1167 only, (1983) "Genetic Probes Become Ever Sharper".
Bauman, et al., J. Histochem. Cytochem, vol. 29:2, pp. 227-238, (1981) "Cytochemical Hybridization with Fluorochrome-labeled RNA".
Landegren, et al., Science, vol. 241, pp. 1077-1080, (1988) "A Ligase-Mediated Gene Detection Technique".
Goldkorn, et al., Nucleic Acids Research, vol. 14:22, pp. 9171-9191, (1986) "A simple and efficient enzymatic method for covalent attachment of DNA to cellulose".
Bischofberger, et al., Nucleic Acids Research, vol. 15:2, pp. 709-716, (1987) "Cleavage of single stranded oligonucleotides by EcoRI restriction endonuclease".
Wiaderkiewiez, et al., Nucleic Acid Res., vol. 15:19, pp. 7831-7848, (1987) "Mismatch and blunt to protruding-end joining by DNA ligases".
Goffin, et al., Nucleic Acid Res., vol. 15:21, pp. 8755-8771, (1987) "Nicks 3' or 5' to AP sites or to mispaired bases, and one-nucleotide gaps can be sealed by T4 DNA ligase".
Krupp et al., FEBS Letts 212(2):271-275 (Feb. 1987).
Goodman Thomas C.
Stull Paul D.
Ullman Edwin F.
Bosse Mark L.
Kaku Janet K.
Leitereg Theodore J.
Syntex (U.S.A.) Inc.
Zitomer Stephanie W.
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