Method for detection of Pseudomonas aeruginosa using polymerase

Details Method for detection of Pseudomonas aeruginosa using polymerase Method for detection of Pseudomonas aeruginosa using polymerase

1996-11-12

2000-04-11

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 231, 536 243, 935 76, 935 77, 935 78, C12Q 168, C07H 2102, C07H 2104, C12N 1500

Patent

active

060486880

ABSTRACT:
The present invention relates to a method for detecting the presence of P. aeruginosa in a fluid sample. The method uses PCR to amplify the expression of a segment of the exotoxin A gene sequence. The method includes treating strands of nucleotide fragments with a first oligonucleotide primer and a second oligonucleotide primer. The primers are sufficiently complementary to the fragment to hybridize a region having from about 400 to 1200 base pairs. Desirably, the first oligonucleotide primer includes the sequence 5'-ACA ACG CCC TCA GCA TCA CCA-3' and the second oligonucleotide primer includes the sequence 5'-CGG GTC GAG CAG GCA CAA C-3'.

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