Method for detection of polynucleotide hybridization

Details Method for detection of polynucleotide hybridization Method for detection of polynucleotide hybridization

1995-06-07

1999-08-31

Sisson, Bradley L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

4352872, 536 243, 422 681, C12Q 168, C12M 100, C07H 2104, G01N 1506

Patent

active

059452803

ABSTRACT:
A method for detecting polynucleotide hybridization is disclosed. A polynucleotide is immobilized on a surface of a piezoelectric crystal. The resonance frequency of the piezoelectric crystal is measured through means for determining the resonant frequency of a piezoelectric crystal. A separate source of polynucleotide is exposed to the polynucleotide-coated piezoelectric crystal for a sufficient length of time and under conditions suitable for hybridization. The resonance frequency of the crystal is then again measured, and the difference between the resonance frequency before and after the incubation period will indicate the extent of hybridization.

REFERENCES:
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patent: 4672111 (1987-06-01), Haley et al.
patent: 4847193 (1989-07-01), Richards et al.
Kathryn Ann Drozda, "The use of Hybridization, Piezoelectric Crystals and Recombinant DNA Technology in the Detection of Salmonella typhimurium", masters thesis, University of Southern Mississippi Aug. 1985.
Sommer and Tautz, Nucleic Acids Research, vol. 17, No. 16, 1989, p. 6749.
"Characterization and Clinical Identification of Enterobacteriaceae by DNA Hybridization" by Don J. Brenner, Ph.D., Progress and Clinical Pathology, 1978, Chapter 4.
Sambrook et al., Molecular Cloning a Laboratory Manual, Second Edition, Cold Spring harbor Laboratory Press, pp. 9.47-9.52, 1989.

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