Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving transferase
Patent
1986-03-18
1989-01-31
Nucker, Christine M.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving transferase
435 24, 435184, 435810, C12Q 152
Patent
active
048015350
ABSTRACT:
Disclosed are improvements in methods for determining the presence of suprathreshold amounts of aspartate aminotransferase in an oral fluid sample by (1) incubation of aspartate aminotransferase substrate materials in a first reaction mixture with the sample to form a first reaction product and (2) detection of reaction product therein by incubation with an indicator substance in a second reaction mixture wherein a second colored reaction product is formed. The improvement comprises (a) utilizing a diazonium dyestuff as the indicator substance in the second reaction and (b) modifying reaction conditions within the first and second reaction mixtures such that no visually detectable colored reaction product will be formed in the second reaction mixture by subthreshold level of aspartate aminotransferase but wherein a visually detectable color reaction product will be formed in the reaction mixture when a selected suprathreshold concentration of aspartate aminotransferase is present in the first reaction mixture.
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Babler Scott D.
Baram Peter
Rudolph Paul H.
Waxman Frank J.
Nucker Christine M.
Xytronyx, Inc.
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