Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1992-09-24
1994-12-27
Parr, Margaret
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 911, 435 9132, 436 63, 935 77, 935 78, C12Q 168, C12P 1934, G01N 3350
Patent
active
053765257
ABSTRACT:
A method for detecting mycoplasma infection in a sample by contacting the sample with labeled oligonucleotides, then measuring incorporation (if any) of the label into mycoplasma RNA.
REFERENCES:
Neale (J. Bacteriol (1984) 158: 943-947.
Gebeyehu Nucl Acids Res 1987 15: 4513-4534.
Lau et al Carcinogenesis (1991) 12: 885-893.
Vlasov et al, Bropolim Kletka 1991 7(5) 37.
Van Kuppeveld et al Applied and Environmental Microbiology (1992) 58: 2606-2615.
Sasaki, et al, Microbiol Immunol (1992) 36: 21-28.
Hellung-Larsen, et al., "Influence of Mycoplasma Infection on the Incorporation of Different Precursors into RNA Components of Tissue Culture Cells", Experimental Cell Research 99, pp. 295-300, 1976.
Merkenschlager, et al., "Rate of Incorporation of Radiolabelled Nucleosides does not Necessarily Reflect the Metabolic State of Cells in Culture: Effects of Latent Mycoplasma Contamination", Immunology 63, pp. 125-131, Aug. 27, 1988.
Schneider, et al., "Incorporation of Tritiated Uridine and Tritiated Uracil into RNA, A Simple Technique for the Detection of Mycoplasma Contamination of Cultured Cells", Experimental Cell Research 84, pp. 311-318, 1974.
McIvor, "Differences in Incorporation of Nucleic Acid Bases and Nucleosides by Various Mycoplasma and Acholeplasma Species", Journal of Bacteriology, vol. 133, No. 2, pp. 438-489, Feb. 13, 1978.
Harley, "HeLa Cell Nucleic Acid Metabolism, The Effect of Mycoplasma Contamination", Biochimica et Biophysica ACTA 213, pp. 171-182, Mar. 9, 1970.
Razin, S., "Nucleic Acid Precursor Requirements of Mycoplasma Laidlawii", Journal of General Microbiology, 28, pp. 243-250, 1962.
Hewish, "Incorporation of .sup.32 P into Ribosomal RNA, Transfer RNA and Inositol Hexaphosphate in Germinating Pea Cotyledons", Biochimica et Biophysica Acta, 228, pp. 509-516, 1971.
Trebichavsky, "Incorporation of Radiophosphorus into Nucleic Acids of Frog Embryo", Folia Biologica, pp. 54-58, 1970.
Razin, et al., "Rapid Detection of Mycoplasmal Infection: DNA Probes for Detection and Identification of Mycoplasmas", Israel Journal of Medical Sciences, vol. 23, pp. 735-741, 1987.
Gobel, et al., "Synthetic Oligonucleotide Probes Complementary to rRNA for Group and Species Specific Detection of Mycoplasmas", Israel Journal of Medical Sciences, vol. 23, pp. 742-746, 1987.
Gobel, et al., "Oligonucleotide Probes Complementary to Variable Regions of Ribosomal RNA Discriminate between Mycoplasma Species", Journal of General Microbiology, 133, pp. 1969-1974, 1987.
Mattsson, et al., "Detection of Mycoplasma Bovis and Mycoplasma Agalactiae by Oligonucleotide Probes Complementary to 16s rRNA", Molecular and Cellular Probes, 5, pp. 27-35, 1991.
Plamer, et al., "Development and Evaluation of the Polymerase Chain Reaction to Detect Mycoplasma Genitalium", FEMS Microbiology Letters, 77, pp. 199-203, 1991.
Jensen, et al., "Polymerase Chain Reaction for Detection of Mycoplasma Genitalium in Clinical Samples", Journal of Clinical Microbiology, vol. 29, No. 1, pp. 46-50, Jan. 1991.
Harasawa, et al., "Detection of Mycoplasma Hypopneumoniae DNA by the Polymerase Chain Reaction", Molecular and Cellular Probes, 5, pp. 103-109, 1991.
Blanchard, et al., "Detection and Identification of Mycoplasmas by Amplification of rDNA", FEMS Microbiology Letters, 81, pp. 37-42, 1991.
Geselowitz Daniel A.
Neckers Leonard M.
Olson Lyn D.
Myers Carla
Parr Margaret
The United States of America as represented by the Department of
LandOfFree
Method for detection of mycoplasma does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for detection of mycoplasma, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for detection of mycoplasma will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-918642