Method for detection of multiple test materials in a sample

Details Method for detection of multiple test materials in a sample Method for detection of multiple test materials in a sample

2007-03-23

2009-06-09

Chunduru, Suryaprabha (Department: 1637)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C536S024310, C536S024320

Reexamination Certificate

active

07544474

ABSTRACT:
A method for sequentially detecting multiple target nucleic acid fragments in a sample includes steps of adding a sample into a column having a test snare which has thereon two or more single strand capture DNA sequences; wherein each capture sequence binds specifically with one target nucleic acid fragment, and forms a double strand segment; washing out unbound target nucleic acid fragment; adding a first DNA probe, which has thereon a chemical label, to attach specifically to a probe binding site of the first target nucleic acid fragment; washing out unbound first probe; adding a triggering solution to trigger the chemical label; and detecting signals on the test snare for determining the first target nucleic acid fragment; subsequently, adding a second DNA probe to bind specifically to the second target nucleic acid fragment; washing, triggering and detecting signals for determining the second target nucleic acid fragment in the same manner.

REFERENCES:
patent: 5804384 (1998-09-01), Muller et al.
patent: 6174733 (2001-01-01), Chen
patent: 6337214 (2002-01-01), Chen
patent: 7195867 (2007-03-01), Chen

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