Method for detection of microorganisms

Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is particulate and the particles are of...

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Details

435 793, 435973, 436533, 436534, 436536, 436538, 436541, 436800, 436824, G01N 33544, G01N 33545, G01N 33546

Patent

active

052907074

ABSTRACT:
A microorganism detection system provides initial warning, confirmation of dentity, and recognition of pathogenic factors in microorganisms from environmental samples. The method and apparatus of the invention uses different sized antibody coated microspheres which react with unknown antigens, are sized by electronic volume sizing, and are sorted by size. The sized particles are quantitated in addition to being sized. The microsphere sizes indicate the presence of specific microorganism groups.
The samples can be further analyzed using fluorescent microspheres which agglutinate with the sized microspheres. The presence of specific microorganisms is indicated by a change in the fluorescence of the sample.

REFERENCES:
patent: 4184849 (1980-01-01), Cambiaso et al.
patent: 4305925 (1981-12-01), Kapmeyer et al.
patent: 4665020 (1987-05-01), Saunders
patent: 5071774 (1991-12-01), Vorpahl et al.

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