Method for detection of base sequence of interest

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C536S023100, C536S024100

Reexamination Certificate

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10802055

ABSTRACT:
There is provided a method for the detection of a base sequence of interest when amount of a sample DNA or RNA is little and plural base sequences of interest to be detected are present in the sample DNA or RNA.The Problem is solved by a method for the detection of an base sequence of interest in a sample DNA or RNA comprising the steps of (1) contacting a sample DNA or RNA to a probe DNAs or RNAs in an aqueous solution to form a hybridization complex; (2) isolating the hybridization complex; (3) dissociating the complex to recover the probe DNAs or RNAs; and (4) identifying the said probe DNAs or RNAs to detect an base sequence of interest in the sample DNA or RNA.

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Veerle A.M.C. Somers, et al. “A rapid, reliable method for detection of known point mutations: Point-EXACCT”,Nucleic-Acids Research, vol. 22, No. 22, Nov. 11, 1994, pp. 4840-4841, XP002013669.
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Mark Schena, et al. “Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray”Science, vol. 270, pp. 467-470, Oct. 20, 1995.

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