Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving luciferase
Patent
1988-05-20
1993-11-02
Saunders, David
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving luciferase
435 4, 435259, 435291, 435311, 436807, 422 52, 422 81, 422 82, 422 8205, C12Q 166
Patent
active
052582850
ABSTRACT:
The number of bacteria in a cell population comprising bacteria and somatic cells is determined by concentrating the bacteria and the somatic cells on the surface of a substrate of a filter material, such as a membrane filter, rupturing the somatic cells by treatment with a medium which is not in osmotic balance with the medium inside the cells, such as water which here constitutes a hypotonic medium, and removing or inactivating their ATP, establishing an extraction chamber having an inner wall which is partly defined by the bacteria-bearing surface of the filter material and therein extracting the ATP from the bacteria with a medium containing a surfactant, such as a quaternary ammonium compound, capable of perforating the cell walls of the bacteria, adding luciferin and luciferase and transferring the medium to a measuring chamber, determining the amount of ATP by measuring the light emitted as a result of the luciferin/luciferase reaction and expressing the amount of ATP as the number of bacteria present on the substrate.
REFERENCES:
patent: 3616253 (1971-10-01), D'Eustachio
patent: 3745090 (1973-07-01), Chappelle et al.
patent: 3971703 (1976-07-01), Picciolo et al.
patent: 4013418 (1977-03-01), Plakas
patent: 4144134 (1979-03-01), Plakas
patent: 4283490 (1981-08-01), Plakas
patent: 4303752 (1981-12-01), Kolahmainan et al.
patent: 4385113 (1983-05-01), Chappelle et al.
patent: 4421848 (1983-12-01), Whitlock
Darnall et al. Molecular Cell Biology Scientific American Books: New York (1986), pp. 167-169.
Jensen Acta Pharmacol. Toxicol. 38: 465-73, 1976.
Davies et al. J. Appl. Bacteriol. 31: 448-61, 1968.
Practical Immunology, Third Edition (1989) Hudson et al, p. 469 European Office Action of May 7, 1991.
Proceedings of Soc. for Experimental Biol. & Medicine, vol. 183, 1986, pp. 74-80, T. S. Tsai et al.
Rapid Methods & Automation in Microbiology; pp. 162-165, May 1981, H. H. Johnston et al.
International Symposium on Analytical Applns of Bioluminescence and Chemiluminescence, pp. 446-447, 1980, H. H. Johnston et al.
A/S Foss Electric Holding
Chin Christopher L.
Saunders David
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