Method for detection of amplified nucleic acid products and rela

Details Method for detection of amplified nucleic acid products and rela Method for detection of amplified nucleic acid products and rela

1991-11-08

1999-02-16

Arthur, Lisa B.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

935 77, 935 78, C12Q 168, C12P 1934

Patent

active

058719067

ABSTRACT:
Disclosed is a method for detecting the presence or absence of amplified nucleic acid products. The method comprises the addition of hapten-labeled nucleotide or target molecule-labeled nucleotide to an amplification reaction and after the amplification process assaying for the presence of nucleic acid products having incorporated hapten-labeled nucleotide or target molecule-labeled nucleotide. Immobilization and detection of the label is accomplished using an affinity molecule that binds specifically to the hapten or target molecule. Thus, detection of amplified nucleic acid products is direct and does not require hybridization with a labeled specific probe.

REFERENCES:
patent: 4851331 (1989-07-01), Vary et al.
patent: 4965188 (1990-10-01), Mullis et al.

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