Method for detection of a nucleic acid using particulate reagent

Details Method for detection of a nucleic acid using particulate reagent Method for detection of a nucleic acid using particulate reagent

1992-10-30

1994-07-19

Spiegel, Carol A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving virus or bacteriophage

435 6, 435 75, 435 792, 435 794, 435 795, 435962, 435969, 435970, 435974, 435975, 436518, 436523, 436531, 524817, 524825, 526286, 526293, 526320, 526346, 935 77, 935 78, C12Q 170, G01N 2100, C08L 3300, C08F 1216

Patent

active

053308913

ABSTRACT:
Biologically active reagents are prepared from particles of copolymers having polyoxyalkylene side chains, each of which side chains has a molecular weight of at least about 88. The reagents are prepared by covalently attaching biologically active substances, for example antibodies, to the particles, directly or indirectly through reactive groups on the particle surface. These reagents are used to advantage in analytical elements and methods for the detection of specific binding ligands (such as immunological species) and immunoassays, and in purification methods as affinity chromatography reagents. Adsorption of undesirable proteins on the particles of the reagents was considerably reduced because of the specific composition of the particles.

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