Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1990-06-20
1991-12-24
Nucker, Christine
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 6, 435 91, 4351723, 435803, 536 27, 436 63, 436 94, 436501, 436504, 935 2, 935 10, 935 77, 935 78, C12Q 170, C12Q 168
Patent
active
050752138
ABSTRACT:
A DNA probe has been isolated which is capable of hybridizing to an oligonucleotide sequence coding for a polypeptide from a major 64 Kilodalton protein of human cytomegalovirus (HCMVgp64). The probe has a sequence of at least seventeen (17) to as many as seven hundred twenty-one 721) nucleotides. The probe may be labelled as by radioactivity. The probe has been used to screen DNA fragments constituting a subgenomic library of human cytomegalovirus DNA to obtain DNA fragments coding for the major late protein of human cytomegalovirus. The DNA fragments coding for the major late protein of human cytomegalovirus (HCMVgp64) may be hybridized to DNA fragments of HCMV DNA from an individual having human cytomegalovirus infection. The viral DNA can be used as whole HCMV DNA or as fragments formed by digesting the human cytomegalovirus DNA with a restriction endonuclease such as one of the restriction endonucleases EcoRI, BamHI, XbaI, HindIII and PrtI. During the screening of clinical specimens, the DNA fragment coding for HCMVgp64 of human cytomegalovirus hybridizes to whole HCMV DNA or to a particular one of the DNA fragments produced by digesting the human cytomegalovirus DNA with the restriction endonucleases. As a resuslt of such hybridization, the identity of human cytomegalovirus may be established. The particular DNA fragment may have a map location of approximately 0.50 to 0.51 units in the human cytomegalovirus (strain Towne) genome. It may have a sequence of about 721 nucleotides. The major late protein of human cytomegalovirus (HCMVgp64) also reacts with T-lymphocytes of an individual after natural infection of that individual with human cytomegalovirus. Thus, the HCMVgp64 protein may be used as a vaccine to prevent HCMV infection.
REFERENCES:
patent: 4358535 (1982-11-01), Falkow et al.
patent: 4762780 (1988-08-01), Spector et al.
Nowak, B. et al., Virology 134 (Apr. 1984), 91-102.
Ruger, B. et al., J. Virology 61 (Feb. 1987), 446-453.
Irmiere, A. et al., Virology 130 (Oct. 1983), 118-133.
Pande, H. et al., Proc. Nat'l. Acad Sci. USA, vol. 81, No. 15, 1984, pp. 4965-4969.
Clark, B. R. et al., J. Virology, vol. 49, No. 1, 1984, pp. 279-282.
Stinski, M. F. et al., J. Virology, vol. 46, No. 1, 1983, pp. 1-14.
Gelmann, E. P. et al., Proc. Nat'l. Acad. Sci. USA, vol. 80, 1983, pp. 5107-5111.
Fleckenstein, B. et al., Gene, vol. 18, 1982, pp. 39-46.
Tamashiro, J. C. et al., J. Virology, vol. 42, No. 2, 1982, pp. 547-557.
Spector, S. A., The Lancet, Feb. 19, 1983, pp. 378-380.
Pande Hema
Riggs Arthur D.
Zaia John A.
City of Hope
Irons Edward S.
Nucker Christine
Scheinek Laurie
LandOfFree
Method for detection and prevention of human cytomegalovirus inf does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for detection and prevention of human cytomegalovirus inf, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for detection and prevention of human cytomegalovirus inf will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-42918