Method for detection and identification of Anacardium...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C435S091200, C435S091400

Reexamination Certificate

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06942976

ABSTRACT:
This invention relates toAnacardiumsp. specific genomic DNA sequence and the methods for utilization of these sequences in detection of Cashew husk in tea samples. Particularly this invention relates to a very sensitive, accurate and efficient method of identification ofAnacardium occidentale(cashew) species. The method is designed to detect presence of any part of cashew plant including the dried and ground apple in market samples of made tea. The main application of this invention is to detect the adulteration of loose as well as branded tea by any part of cashew plant and thus is a part of quality control measures, in addition to the taxonomical authentication of cashew plants.

REFERENCES:
Garg, “Substitute and Adulterant Plants,”Periodical Experts Book Agency, New Delhi, India, pp. 136-142, 1992.
Sudershan et al., “Changing Profile of Food Adulteration: Perception of Food Analysis,”J. Food Sci. Technol., vol. 32, No. 5, pp. 368-372, 1995.
Martsinlovskaya et al., “Potential Use of PCR-Amplified Ribosomal Intergenic Sequences for Differentiation of Varieties and Species ofGossypiumCotton,”Plant Molecular Biology Reportervol. 14, No. 1, pp. 44-49, 1996.
Shingh et al., “Isolation and PCR Amplification of Genomic DNA from Market Samples of Dry Tea,”Plant Molecular Biology Reporter, vol. 17, pp. 171-178, 1999.
Silva et al.,Acta Horticulturae, No. 370, pp. 20-26, 1995.
Anonymous, 1999-III,The Assam Review and Tea News, vol. 87, pp. 28-29, 1999.
Anonymous, 1999-II,Divya Himachal, 1999.

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