Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2001-12-20
2008-12-02
Horlick, Kenneth R. (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091200
Reexamination Certificate
active
07459271
ABSTRACT:
The invention is a method for detecting whether an organism is homozygous or heterozygous in a target nucleotide. The method combines hybridization of two differently labelled probes A and B on either side of a restriction site, restriction endonuclease digestion, and detection of the labels in the resulting digest. If an organism is: homozygous then the labels will be present in the ratio 1:1 or 1:0; or if heterozygous then the labels will be present in the ratio 1:2. Either immobilized array technology or electrophoretic separation may be used.
REFERENCES:
patent: 6004783 (1999-12-01), Ausubel et al.
patent: 6110709 (2000-08-01), Ausubel et al.
patent: 2003/0175729 (2003-09-01), Van Eijk et al.
patent: 2 718 461 (1995-10-01), None
patent: 9325563 (1993-12-01), None
patent: WO 98/12352 (1998-03-01), None
patent: 9853098 (1998-11-01), None
patent: 9931272 (1999-06-01), None
Daly et al., Novel polymorphism in the FMR1 gene resulting in a “pseudodeletion” of FMR1 in a commonly used fragile X assay. J. Molec. Diag. (2000) 2:128-131.
Bathum, L. et al., “A Dual Label Oligonucleotide Ligation Assay for Detection of the CYP2C19*1, CYP2C19*2, and CYP2C19*3 Alleles Involving Time-Resolved Fluorometry,”Therapeutic Drug Monitoring 20(1):1-6, 1998.
Finch, J. et al., “PCR/RFLP Assay for Copy Number of Mutant and Wild-Type Alleles,”BioTechniques 21(6):1055-1060, 1996.
Nikiforov, T. et al., “The Use of Phosphorothioate Primers and Exonuclease Hydrolysis for the Preparation of Single-stranded PCR Products and their Detection by Solid-phase Hybridization,”PCR Methods Appl., 3:285-291, 1994.
Shi, M. et al., “Technologies for Detecting Genetic Polymorphisms in Pharmacogenomics,”Molecular Diagnosis, 4(4):343-351, 1999.
Shinder et al., A Sensitive Restriction Fragment Length Polymorphism Method to Detect CAA-AAA Mutations at Codon 61 of Ha-ras, Molecular Carcinogenesis, 1993, 263-267, vol. 7.
Horlick Kenneth R.
Murdoch Childrens Research Institute
Seed IP Law Group PLLC
Thomas David C
LandOfFree
Method for detecting whether an organism is homozygous or... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for detecting whether an organism is homozygous or..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for detecting whether an organism is homozygous or... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4041816