Method for detecting total ketone bodies in urine

Details Method for detecting total ketone bodies in urine Method for detecting total ketone bodies in urine

1996-03-19

1998-09-01

Alexander, Lyle A.

Chemistry: analytical and immunological testing

Oxygen containing

Carbonyl, ether, aldehyde or ketone containing

436164, 436 48, G01N 33493

Patent

active

058010599

ABSTRACT:
Aliquot of a urine sample is placed in a first automated analyzer sampling cup and a known standard is placed in a second cup. The urine sample and standard are transferred to separate cuvettes and at least one reagent composition in an aqueous medium is injected into the cuvette. The reagent composition contains a compound to remove substances in the urine interfering with a calorimetric reaction, a compound to convert B-hydroxybutyric acid in the urine to acetoacetic acid in the presence of nicotinamide adenine dinucleotide and reading at a specified wavelength to determine quantitatively the total ketone bodies in the patient's urine.

REFERENCES:
patent: 5326697 (1994-07-01), Magers
patent: 5510245 (1996-04-01), Magers

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