Method for detecting the presence of peroxidatively active subst

Details Method for detecting the presence of peroxidatively active subst Method for detecting the presence of peroxidatively active subst

1991-03-18

1993-01-26

Housel, James C.

Chemistry: analytical and immunological testing

Hemoglobin, myoglobin, or occult blood

435 28, 422 56, G01N 3372

Patent

active

051822130

ABSTRACT:
An assay for the detection of a peroxidatively active substance in a test sample. The assay employs a phenylenediamine and a naphthol which, under the conditions of the assay, couple to form a chromogen thereby indicating the presence of the analyte. The assay is carried out at a pH of from 10 to 14 since false positives due to ascorbate interference are minimized in highly alkaline media.

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