Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1976-09-10
1980-01-08
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
C12K 104, G01N 2778, G01N 3316
Patent
active
041826566
ABSTRACT:
A sample of material to be tested for the presence of biologically active agents, such as bacteria, is introduced into a sealable container partially filled with a culture medium comprising a .sup.13 C-labeled fermentable substrate, the remainder of the container being filled with a culture gas, the container and its contents are subjected to conditions conducive to biological activity for a predetermined period sufficient for fermentation of the medium to produce carbon dioxide after which the ratio of .sup.13 CO.sub.2 to .sup.12 CO.sub.2 in the culture gas in the container is determined and compared to the initial ratio of .sup.13 CO.sub.2 to .sup.12 CO.sub.2 in the culture gas in order to detect any differences indicating the presence of biologically active agents in the sample.
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Ahnell Joseph E.
Broman Rodney L.
Waters John R.
Johnston Laboratories, Inc.
Wiseman Thomas G.
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