Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1984-05-25
1989-07-18
Warden, Robert J.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 18, 435 21, 435 29, 4351721, 435849, 43525233, 935 82, C12Q 168, C12Q 134, C12Q 142, C12Q 102
Patent
active
048493350
ABSTRACT:
A method for the detection of the mutagen effect of a substance or composition by producing culture of a micro-organism harboring a recombinant of a sfi gene and of a gene coding for a dosable enzyme in the presence of said substance or composition and measuring the activity of the dosable enzyme induced under the control of the sfi gene. When the latter is activated due to the mutagen character of said substance or composition, measuring the activity of a distinct enzyme synthesized by the microorganism and coded by a gene not involved in the activation process of the sfi gene and measuring the variation of the ratio of the activity of the abovesaid dosable enzyme to the activity of said distinct enzyme. Then comparing the variation that of the ratio of the activities of the same enzyme in a culture of the same microorganism, but in the absence of the mutagen substance.
REFERENCES:
Quillardet et al., "Biochimie", vol. 64 (1982), pp. 797-801.
Quillardet et al, Proc. Natl. Acad. Sci., vol. 79, pp. 5971-5975 (Oct. 1982) [not prior art].
Huisman and Ari, Nature, vol. 290, pp. 797-799 (Apr. 30, 1981).
D'Ari Richard
Hofnung Maurice
Huisman Olivier
Perrin David
Quillardet Philippe
Deck Randall E.
Institut Pasteur
Warden Robert J.
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