Method for detecting target substance utilizing probe...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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Reexamination Certificate

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07655407

ABSTRACT:
It is an object of the present invention to provide a method for detecting a target substance in a specimen with the use of fine particles, whereby the target substance can be readily detected with the exclusive use of a single type of probe and the detection limit is improved. The present invention provides a method for detecting a target substance in a specimen which comprises the steps of: allowing a complex of a fine particle and a probe to come into contact with a specimen; and detecting changes in physical properties of the fine particle that are caused by desorption of the probe from the fine particle due to interaction between the target substance in the specimen and the probe.

REFERENCES:
patent: 6506564 (2003-01-01), Mirkin et al.
patent: 2008/0176263 (2008-07-01), Schultz et al.
Jiang et al., “Gold-Labeled Nanoparticle-Based Immunoresonance Scattering Spectral Assay for Trace Apolipoprotein AI and Apolipoprotein B,” Clinical Chemistry, 2006, vol. 52, No. 7, pp. 1389-1394.
Elghanian et al., “Selective Colorimetric Detection of Polynucleotides Based on the Distance-Dependent Optical Properties of Gold Nanoparticles,” Science, Aug. 1997, vol. 277, pp. 1078-1081.

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