Method for detecting target nucleic acid in specimen

Details Method for detecting target nucleic acid in specimen Method for detecting target nucleic acid in specimen

1992-09-16

1997-02-11

Fleisher, Mindy B.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 2433, 435 912, 935 77, 935 78, C12Q 168, C07H 2104, C12P 1934, C12N 1500

Patent

active

056019769

ABSTRACT:
An intended nucleic acid is determined in a sample by a method in which at least one of the nucleic acid strands of the intended nucleic acid is subjected to hybridization with a primer and to chain-extension reaction over the primer to form a synthesized nucleic acid which is complementary to and in hybridization with the strand; a copy of the intended nucleic acid is obtained, which copy is (a) the nucleic acid of the double-stranded double produced by the chain-extension reaction, or (b) the synthesized nucleic acid freed from the double-stranded nucleic acid, or (c) a double stranded nucleic acid form from a pair of the synthesized nucleic acids complementary with each other; and it is determined whether the copy is present in the sample thereby to know whether the intended nucleic acid is in present in the sample.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4806546 (1989-02-01), Carrico et al.
patent: 4851331 (1989-07-01), Varey et al.
patent: 4965188 (1990-10-01), Mullis et al.
patent: 4968602 (1990-11-01), Dattagupta

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