Method for detecting Staphylococcus aureus

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

Reexamination Certificate

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C435S006120, C536S024320, C536S024300, C536S023100

Reexamination Certificate

active

06946267

ABSTRACT:
Specific nucleic acid sequences, e.g., SEQ ID NOs:2-8, for detectingStaphylococcus aureus. Also disclosed is a method of detectingStaphylococcus aureus. The method includes providing a sample having a nucleic acid from an unknown microorganism; amplifying the nucleic acid with a pair of primers, each containing an oligo-nucleotide selected from theStaphylococcus aureusgap regulator gene region (SEQ ID NO:1) and each having 14-40 nucleotides in length; and detecting an amplification product. Detection of the amplification product, e.g., using SEQ ID NO:2, 7, or 8 as a probe, indicates the presence ofStaphylococcus aureus.

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