Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1993-07-06
1996-05-07
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 6, 435 912, C12Q 170, C12Q 168, C12P 1934
Patent
active
055145400
ABSTRACT:
A method for detecting specific nucleic acid sequences (a single sequence and/or a mixture of nucleic acid sequences) which are present in a biological sample, comprising at least one enzymatic amplification. Detection is carried out, after placing the biological samples in a solution in order to extract the nucleic acid, by enrichment with target sequences by putting the biological samples into contact with at least one pair of appropriate triggers, followed by at least one appropriate dilution of the obtained enrichment amplifying solution, and by putting a fraction of this solution into contact with at least one pair of triggers, followed by the detection of the obtained double-strand target nucleic acid copies. Applications in the diagnosis of genetic, infectious and tumoral diseases, in checking biological samples and in cell typing etc.
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Fouque Brigitte
Livache Thierry
Sauvaigo Sylvie
Teoule Robert
Cis Bio International
Horlick Kenneth R.
Jones W. Gary
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