Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2005-12-06
2005-12-06
Fredman, Jeffrey (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091200, C536S023100
Reexamination Certificate
active
06972174
ABSTRACT:
A method of genotyping single nucleotide polymorphisms (“SNP”) and point mutations in nucleic acid based on chain extension by polymerase. This invention is based on the fact that the nucleoside immediately 5′ adjacent to any SNP/point mutation site is known, and the neighboring sequence immediately 3′ adjacent to the site is also known. A primer complementary to the sequence directly adjacent to the SNP on the 3′ side in a target polynucleotide is used for chain elongation. The polymerase reaction mixture contains one chain-terminating nucleotide having a base complementary to the nucleotide directly adjacent to the SNP on the 5′ side in the target polynucleotide. An additional dNTP may be added to produce a primer with the maximum of a two-base extension. The resultant elongation/termination reaction products are analyzed for the length of chain extension of the primer, or for the amount of label incorporation from a labeled form of the terminator nucleotide.
REFERENCES:
patent: 6110709 (2000-08-01), Ausubel et al.
patent: 6479242 (2002-11-01), Guo et al.
patent: WO 93/02212 (1993-02-01), None
patent: WO 00/20853 (2000-04-01), None
Sun et al (Nucleic Acids Research (Jun. 15, 2000) 28(12):e68, p. i-viii).
Okano et al (Sensors and Actuators B (2000) 64:88-94.
Piggee et al (J. Chromatog. A (1997) 781:367-375.
J. Sambrook, et al., Molecular Cloning A Laboratory Manual, Second Edition, 1989, pp. 13.65-13.69, Cold Spring Harbor Laboratory Press.
Ausubel, et al., Current Protocols in Molecular Biology, 1998, pp. 7.4A. 13-7.4A. 19, vol. 1, John Wiley & Sons.
Wong Jeffrey Tze-Fei
Xue Hong
Fredman Jeffrey
Jackson Walker L.L.P.
PharmacoGenetics, Ltd.
LandOfFree
Method for detecting single nucleotide polymorphisms (SNP's)... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for detecting single nucleotide polymorphisms (SNP's)..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for detecting single nucleotide polymorphisms (SNP's)... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3492225