Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2005-03-08
2005-03-08
Benzion, Gary (Department: 1634)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091100, C536S023100, C536S024300, C536S024330, C536S025300
Reexamination Certificate
active
06864056
ABSTRACT:
The present invention concerns a method for detecting the presence of a ribosome inactivating protein in a sample. The method comprises contacting a sample suspected of containing a ribosome inactivating protein with a 2′-O-methylated oligonucleotide substrate having a GAxGA tetraloop wherein “Ax” is a deoxyribonucleoside comprising a fluorescent adenine derivative or analog base capable of emitting a fluorescence when released from the nucleoside, “Ax”, and detecting the presence of the fluorescent adenine derivative or analog base released from the tetraloop as an indication of the presence of the ribosome inactivating protein in the sample. The present invention is also directed to an assay kit and a reagent useful for carrying out the steps of the above method.
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patent: 6034233 (2000-03-01), Ecker et al.
Kao et al., Novel rapid method for the detection of ricin A-chain and related ribotoxins. Proceedings of the ERDEC Scientific Conference on Chemical and Biological Defense Research, pp. 483-491, on Nov. 17-20, 1998, MD, United States.*
Zamboni et al., High-pressure-liquid-chromatographic and fluorimetric methods for the determination of adenine released from ribosomes by ricin and gelonin. Biochem. J., 259, 639-643, 1989.*
Szewczak et al., The conformation of the sarcin/ricin loop from 28S ribosomal RNA. Proc. Natl. Acad. Sci. USA, 90, 9581-9585, Oct. 1993.*
Brigotti et al., A rapid and sensitive method to measure the enzymatic activity of ribosome-inactivating proteins. Nucleic Acids Res., 26, 4306-4307, 1998.
Davies Julian E.
Kao Richard
Young Ronald
Benzion Gary
Biffoni Ulysses John
Lu Frank W
The United States of America as represented by the Secretary of
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