Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2006-03-07
2006-03-07
Horlick, Kenneth R. (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S091100, C435S091200, C536S023100
Reexamination Certificate
active
07008768
ABSTRACT:
A method is disclosed for detecting exposure of organisms to biologically significant or hazardous amounts of ionizing radiation. The method uses nucleic acid microarray hybridization to evaluate biological effects, such as patterns of expression of genes after radiation exposure. Numerous genes are provided which have been found to be responsive to radiation exposure in a variety of cell lines. These genes are incorporated into probe sets, which are exposed to a labeled nucleic acid composition from a test cell, such as cDNA reverse transcribed from mRNA in the test cell, which specifically hybridizes to members of the probe set when the cell has been exposed to a biologically significant amount of ionizing radiation. Whether the nucleic acid composition hybridizes to the nucleic acid molecules representing genes that are differentially expressed is determined. The invention also includes methods for determining a dose response relationship between radiation exposure and differential expression of one or more genes, for example to determine a probable radiation dose in cells that have actually or potentially been exposed to the ionizing radiation. The invention also includes probe sets and microarrays used in this method.
REFERENCES:
patent: 5563060 (1996-10-01), Hozier
patent: 5691157 (1997-11-01), Gong et al.
patent: 5707807 (1998-01-01), Kato
patent: 5744305 (1998-04-01), Fodor et al.
patent: 5770722 (1998-06-01), Lockhart et al.
patent: 5830645 (1998-11-01), Pinkel et al.
patent: 5843655 (1998-12-01), McGall
patent: 5866330 (1999-02-01), Kinzler et al.
patent: 5877215 (1999-03-01), McClay et al.
patent: 5910309 (1999-06-01), Ullrich
patent: 5965352 (1999-10-01), Stoughton et al.
patent: 5998136 (1999-12-01), Kamb
patent: WO 97/30179 (1997-08-01), None
patent: WO 98/18961 (1998-05-01), None
patent: WO 98/56954 (1998-12-01), None
patent: WO 99/55913 (1999-11-01), None
Amundson et al., “Identification of Gamma-Ray Responsive Genes by cDNA Array Hybridization,”Proceedings of the American Association for Cancer Research Annual Meeting39:454 (1998).
Amundson et al., “Induction of Stress Genes by Low Doses of Gamma Rays,”Radiat, Res.152:225-231 (1999).
Amundson et al., “Fluorescent cDNA Microarray Hybridization Reveals Complexity and Heterogeneity of Cellular Genotoxic Stress Responses,”Oncogene18:3666-3672 (1999).
Carulli et al., “High Throughput Analysis of Differential Gene Expression,”J. Cell. Biochem.Suppl.30/31:286-296 (1998).
Derisi et al., “Use of a cDNA Microarray to Analyse Gene Expression Patterns in Human Cancer,”Nature Genetics14:457-460 (1996).
Fiscella et al., “Wipl. A Novel Human Protein Phosphatase that is Induced in Response to Ionizing Radiation in a p53-Dependent Manner,”Proc. Natl. Acad. Sci. USA94:6048-6053 (1997).
Fornace et al., “The Complexity of Radiation Stress Responses: Analysis by Informatics and Functional Genomics Approaches,”Gene Expr.7:387-400 (1999).
Goltry et al., “Induction of Serum Amyloid A Inflammatory Response Genes in Irradiated Bone Marrow Cells,”Radiat. Res.149:570-578 (1998).
Higuchi et al., “Search for Genes Involved in UV-Resistance in Human Cells by mRNA Differential Display: Increased Transcriptional Expression of Nucleophosmin and T-Plastin Genes in Association with the Resistance,”Biochem. Biophys. Res. Comm.248:597-602 (1998).
Iordanov et al., “Ultraviolet Radiation Triggers the Ribotoxic Stress Response in Mammalian Cells,”J. Biol. Chem.273:15794-15803 (1998).
Monks et al., “Feasibility of High-Flux Anticancer Drug Screen Using a Diverse Panel of Cultured Human Tumor Cell Lines,”J. Natl. Cancer Inst.83:757-766 (1991).
Myers et al., “A Protein Expression Database for the Molecular Pharmacology of Cancer,”Electrophoresis18:647-653 (1997).
Prasad et al., “Activation of Nuclear Factor kB in Human Lymphoblastoid Cells by Low Dose Ionizing Radiation,”Radiation Res.138:367-372 (1994).
Schena et al., “Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray,”Science270:467-470 (1995).
Schena et al., “Parallel Human Genome Analysis: Microarray-Based Expression Monitoring of 1000 Genes,”Proc. Natl. Acad. Sci. USA93:10614-10619 (1996).
Weinstein et al., “An Information-Intensive Approach to the Molecular Pharmacology of Cancer,”Science275:343-349 (1997).
Zhang et al., “Differential Display of mRNA,”Mol. Biotech.10:155-165 (1998).
Amundson Sally A.
Fornace, Jr. Albert J.
Trent Jeffrey M.
Horlick Kenneth R.
Kim Young J.
Klarquist & Sparkman, LLP
The United States of America as represented by the Department of
LandOfFree
Method for detecting radiation exposure does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Method for detecting radiation exposure, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Method for detecting radiation exposure will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3530973