Method for detecting prostate cells

Details Method for detecting prostate cells Method for detecting prostate cells

1997-05-05

1999-01-12

Ketter, James

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 911, 435 912, 536 243, 536 2433, C12Q 168

Patent

active

058586733

ABSTRACT:
There is provided a sensitive multiplex RT-PCR assay for the detection of circulating prostate antigen expressing cells. Multiplex PCR uses multiple sets of primers to concurrently amplify different DNA sequences that can be readily resolved by gel electrophoresis. When applied to blood samples from prostate cancer patients, the nested multiplex RT-PCR can simultaneously detect PSA-expressing cells, PSM-expressing cells, and a ubiquitously expressed internal PCR control gene, glyceraldehyde 3-phosphate dehydrogenase (G3PDH), all within a single reaction.

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